Crystallization and Kinetic Properties of Glutamate Dehydrogenase from Frog Liver.

In the course of preparing carbamyl phosphate synthetase from frog liver, a crystalline protein fraction was obtained which proved to be glutamate dehydrogenase. Because of the considerable interest in this enzyme and the striking differences in certain properties of this enzyme from different animal sources (l-6), it seemed of importance to study the kinetic properties of crystalline frog liver glutamate dehydrogenase. The kinetic properties of crystalline frog liver glutamate dehydrogenase are reported in this paper and are compared with those of crystalline beef liver glutamate dehydrogenase.